Journal: Journal of Immunology Research

Article Title: Involvement of the Dectin-1 Receptor upon the Effector Mechanisms of Human Phagocytic Cells against Paracoccidioides brasiliensis

doi: 10.1155/2019/1529189

Figure Lengend Snippet: Dectin-1 receptor expression (MFI) of monocytes (MO) (a) and neutrophils (PMN) (b) treated or not with IFN- γ , TNF- α , and GM-CSF for 18 hours followed by the Pb265 challenge or not for 4 hours. Data are expressed as the mean of 8 healthy volunteer donors tested. ∗ Statistical significance between groups is indicated ( ∗ p < 0.05 × MO control).

Article Snippet: In some cultures, two hours before the challenge with the fungus, cells were incubated or not with 10 μ L (3 μ g/mL) of anti-Dectin-1 monoclonal antibody (monoclonal anti-human Dectin-1/CLEC7A antibody, R&D Systems), to block the receptor and consequently evaluate the Dectin-1 role in P . brasiliensis recognition, fungicidal activity, and H 2 O 2 production by both types of cells.

Techniques: Expressing

Journal: Journal of Immunology Research

Article Title: Involvement of the Dectin-1 Receptor upon the Effector Mechanisms of Human Phagocytic Cells against Paracoccidioides brasiliensis

doi: 10.1155/2019/1529189

Figure Lengend Snippet: Histogram of a representative experiment for Dectin-1 receptor blockage for monocytes (a) and neutrophils (b), showing MFI of monocytes or neutrophil cultures (blue line) or monocytes or neutrophils treated with 3.0 μ g/mL (orange line) of anti-Dectin-1 monoclonal antibody. The red line represents MFI of the isotypic control.

Article Snippet: In some cultures, two hours before the challenge with the fungus, cells were incubated or not with 10 μ L (3 μ g/mL) of anti-Dectin-1 monoclonal antibody (monoclonal anti-human Dectin-1/CLEC7A antibody, R&D Systems), to block the receptor and consequently evaluate the Dectin-1 role in P . brasiliensis recognition, fungicidal activity, and H 2 O 2 production by both types of cells.

Techniques:

Journal: Journal of Immunology Research

Article Title: Involvement of the Dectin-1 Receptor upon the Effector Mechanisms of Human Phagocytic Cells against Paracoccidioides brasiliensis

doi: 10.1155/2019/1529189

Figure Lengend Snippet: Percentage of fungal recovery by colony-forming unit (CFU) analysis of monocyte (MO) cultures treated or not with IFN- γ (a), TNF- α (b), and GM-CSF (c) for 18 hours in the presence or absence of the anti-Dectin-1 monoclonal antibody (AD) and challenged with Pb265 for 4 hours. Data are expressed as the mean of 8 healthy volunteer donors tested. Statistical significance between groups is indicated.

Article Snippet: In some cultures, two hours before the challenge with the fungus, cells were incubated or not with 10 μ L (3 μ g/mL) of anti-Dectin-1 monoclonal antibody (monoclonal anti-human Dectin-1/CLEC7A antibody, R&D Systems), to block the receptor and consequently evaluate the Dectin-1 role in P . brasiliensis recognition, fungicidal activity, and H 2 O 2 production by both types of cells.

Techniques:

Journal: Journal of Immunology Research

Article Title: Involvement of the Dectin-1 Receptor upon the Effector Mechanisms of Human Phagocytic Cells against Paracoccidioides brasiliensis

doi: 10.1155/2019/1529189

Figure Lengend Snippet: Percentage of fungal recovery by colony-forming unit (CFU) analysis of neutrophil (PMN) cultures treated or not with IFN- γ (a), TNF- α (b), and GM-CSF (c) for 18 hours in the presence or absence of the anti-Dectin-1 monoclonal antibody (AD) and challenged with Pb265 for 4 hours. Data are expressed as the mean of 8 healthy volunteer donors tested. Statistical significance between groups is indicated.

Article Snippet: In some cultures, two hours before the challenge with the fungus, cells were incubated or not with 10 μ L (3 μ g/mL) of anti-Dectin-1 monoclonal antibody (monoclonal anti-human Dectin-1/CLEC7A antibody, R&D Systems), to block the receptor and consequently evaluate the Dectin-1 role in P . brasiliensis recognition, fungicidal activity, and H 2 O 2 production by both types of cells.

Techniques:

Journal: Journal of Immunology Research

Article Title: Involvement of the Dectin-1 Receptor upon the Effector Mechanisms of Human Phagocytic Cells against Paracoccidioides brasiliensis

doi: 10.1155/2019/1529189

Figure Lengend Snippet: Hydrogen peroxide (H 2 O 2 ) production by monocytes (MO) treated or not with IFN- γ (a), TNF- α (b), and GM-CSF (c) for 18 hours in the presence or absence of the anti-Dectin-1 monoclonal antibody (AD) and challenged or not with Pb265 for 4 hours. Box-and-whisker plot showing data distribution of 8 healthy volunteer donors. Statistical significance between groups is indicated.

Article Snippet: In some cultures, two hours before the challenge with the fungus, cells were incubated or not with 10 μ L (3 μ g/mL) of anti-Dectin-1 monoclonal antibody (monoclonal anti-human Dectin-1/CLEC7A antibody, R&D Systems), to block the receptor and consequently evaluate the Dectin-1 role in P . brasiliensis recognition, fungicidal activity, and H 2 O 2 production by both types of cells.

Techniques: Whisker Assay

Journal: Journal of Immunology Research

Article Title: Involvement of the Dectin-1 Receptor upon the Effector Mechanisms of Human Phagocytic Cells against Paracoccidioides brasiliensis

doi: 10.1155/2019/1529189

Figure Lengend Snippet: Hydrogen peroxide (H 2 O 2 ) production by neutrophils (PMN) treated or not with IFN- γ (a), TNF- α (b), and GM-CSF (c) for 18 hours in the presence or absence of the anti-Dectin-1 monoclonal antibody (AD) and challenged or not with Pb265 for 4 hours. Box-and-whisker plot showing data distribution of 8 healthy volunteer donors. Statistical significance between groups is indicated.

Article Snippet: In some cultures, two hours before the challenge with the fungus, cells were incubated or not with 10 μ L (3 μ g/mL) of anti-Dectin-1 monoclonal antibody (monoclonal anti-human Dectin-1/CLEC7A antibody, R&D Systems), to block the receptor and consequently evaluate the Dectin-1 role in P . brasiliensis recognition, fungicidal activity, and H 2 O 2 production by both types of cells.

Techniques: Whisker Assay